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Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.
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Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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Image Search Results


Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.

Journal: Scientific Data

Article Title: Continental United States climate projections based on thermodynamic modification of historical weather

doi: 10.1038/s41597-023-02485-5

Figure Lengend Snippet: Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.

Article Snippet: Surface Layer , Eta Similarity scheme , Fifth-Generation Penn State/NCAR Mesoscale Model (Revised MM5) , Fifth-Generation Penn State/NCAR Mesoscale Model (Revised MM5) .

Techniques: Aerosol

a) RNA-seq results of indicated genes that mark WT (eGFP+, Neomycin-resistant) and optoWnt (Cry-LRP6c-2A-mCherry+, Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).

Journal: bioRxiv

Article Title: Optogenetic control of Wnt signaling for modeling early embryogenic patterning with human pluripotent stem cells

doi: 10.1101/665695

Figure Lengend Snippet: a) RNA-seq results of indicated genes that mark WT (eGFP+, Neomycin-resistant) and optoWnt (Cry-LRP6c-2A-mCherry+, Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).

Article Snippet: To generate the Brachyury-2A-eGFP donor plasmid (manuscript under revision), DNA fragments of ∼2 kbp in length were PCR-amplified from the endogenous genomic T locus, before and after the stop codon, and were cloned into the OCT4-2A-eGFP donor plasmid (Addgene plasmid #31938).

Techniques: RNA Sequencing Assay, Expressing